Rapid healing of femoral defects in rats with low dose sustained BMP2 expression from PEGDA hydrogel microspheres

TitleRapid healing of femoral defects in rats with low dose sustained BMP2 expression from PEGDA hydrogel microspheres
Publication TypeJournal Article
Year of Publication2013
AuthorsSonnet, C, Simpson, LSC, Olabisi, RM, Sullivan, K, Lazard, ZW, Gugala, Z, Peroni, JF, Weh, MJ, Davis, AR, West, JL, Olmsted-Davis, EA
JournalJournal of Orthopaedic Research
Paginationn/a - n/a
Date Published06/2013
Keywordsbone healing; bone morphogenetic protein; critical size defect; microencapsulation; poly(ethylene glycol) diacrylate
Abstract

Current strategies for bone regeneration after traumatic injury often fail to provide adequate healing and integration. Here, we combined the poly (ethylene glycol) diacrylate (PEGDA) hydrogel with allogeneic "carrier" cells transduced with an adenovirus expressing BMP2. The system is unique in that the biomaterial encapsulates the cells, shielding them and thus suppressing destructive inflammatory processes. Using this system, complete healing of a 5 mm-long femur defect in a rat model occurs in under 3 weeks, through secretion of 100-fold lower levels of protein as compared to doses of recombinant BMP2 protein used in studies which lead to healing in 2-3 months. New bone formation was evaluated radiographically, histologically, and biomechanically at 2, 3, 6, 9, and 12 weeks after surgery. Rapid bone formation bridged the defect area and reliably integrated into the adjacent skeletal bone as early as 2 weeks. At 3 weeks, biomechanical analysis showed the new bone to possess 79% of torsional strength of the intact contralateral femur. Histological evaluation showed normal bone healing, with no infiltration of inflammatory cells with the bone being stable approximately 1 year later. We propose that these osteoinductive microspheres offer a more efficacious and safer clinical option over the use of rhBMP2.

DOI10.1002/jor.22407
Short TitleJ Orthop Res
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