Multilayer Microfluidic Poly(Ethylene Glycol) Diacrylate Hydrogels

TitleMultilayer Microfluidic Poly(Ethylene Glycol) Diacrylate Hydrogels
Publication TypeBook Chapter
Year of Publication2012
AuthorsCuchiara, MP, West, JL
EditorJenkins, G, Mansfield, CD
Book TitleMicrofluidic Diagnostics: Methods and Protocols
Series TitleMethods in Molecular Biology
Pagination387 - 401
PublisherHumana Press
CityTotowa, NJ
ISBN Number978-1-62703-134-9
Keywordshydrogels; microfluidics; poly(ethylene glycol) diacrylate

Development of robust, in vivo like tissues in vitro holds the potential to create regenerative medicine-based therapeutics, provide more physiologically significant preclinical models and supply a pharmacological and toxicological screening platform that reflects in vivo systems in both complexity and function. This protocol describes a simple, robust, multilayer replica molding technique in which poly(dimethylsiloxane) (PDMS) and poly(ethylene glycol) diacrylate (PEGDA) are serially replica molded to develop microfluidic PEGDA hydrogel networks embedded within independently fabricated PDMS housings, using a combination of soft and photo-lithography. This work has direct applications toward the development of robust, complex, cell-laden hydrogels for in vitro diagnostics and regenerative medicine applications.

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