Protease-activated quantum dot probes

TitleProtease-activated quantum dot probes
Publication TypeJournal Article
Year of Publication2005
AuthorsChang, E, Miller, JS, Sun, J, Yu, WW, Colvin, VL, Drezek, RA, West, JL
JournalBiochemical and Biophysical Research Communications
Pagination1317 - 1321
Date Published9/2005
KeywordsBioimaging; Collagenase; Fluorescence resonance energy transfer; Gold colloid; Nanoparticle probe; Optical contrast agent; Optical quenching; Protease imaging; Proteolytic activity; Quantum dot

We have developed a novel nanoparticulate luminescent probe with inherent signal amplification upon interaction with a targeted proteolytic enzyme. This construct may be useful for imaging in cancer detection and diagnosis. In this system, quantum dots (QDs) are bound to gold nanoparticles (AuNPs) via a proteolytically degradable peptide sequence to non-radiatively suppress luminescence. A 71% reduction in luminescence was achieved with conjugation of AuNPs to QDs. Release of AuNPs by peptide cleavage restores radiative QD photoluminescence. Initial studies observed a 52% rise in luminescence over 47 h of exposure to 0.2 mg/mL collagenase. These probes can be customized for targeted degradation simply by changing the sequence of the peptide linker.

Short TitleBiochem Biophys Res Commun
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