Protease-activated quantum dot probes

TitleProtease-activated quantum dot probes
Publication TypeJournal Article
Year of Publication2005
AuthorsChang, E, Miller, JS, Sun, J, Yu, WW, Colvin, VL, Drezek, RA, West, JL
JournalBiochemical and Biophysical Research Communications
Volume334
Issue4
Pagination1317 - 1321
Date Published9/2005
ISSN0006291X
KeywordsBioimaging; Collagenase; Fluorescence resonance energy transfer; Gold colloid; Nanoparticle probe; Optical contrast agent; Optical quenching; Protease imaging; Proteolytic activity; Quantum dot
Abstract

We have developed a novel nanoparticulate luminescent probe with inherent signal amplification upon interaction with a targeted proteolytic enzyme. This construct may be useful for imaging in cancer detection and diagnosis. In this system, quantum dots (QDs) are bound to gold nanoparticles (AuNPs) via a proteolytically degradable peptide sequence to non-radiatively suppress luminescence. A 71% reduction in luminescence was achieved with conjugation of AuNPs to QDs. Release of AuNPs by peptide cleavage restores radiative QD photoluminescence. Initial studies observed a 52% rise in luminescence over 47 h of exposure to 0.2 mg/mL collagenase. These probes can be customized for targeted degradation simply by changing the sequence of the peptide linker.

DOI10.1016/j.bbrc.2005.07.028
Short TitleBiochem Biophys Res Commun
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