Modification of polyurethaneurea with PEG and YIGSR peptide to enhance endothelialization without platelet adhesion

TitleModification of polyurethaneurea with PEG and YIGSR peptide to enhance endothelialization without platelet adhesion
Publication TypeJournal Article
Year of Publication2004
AuthorsJun, H-W, West, JL
JournalJournal of Biomedical Materials Research
Volume72B
Issue1
Pagination131 - 139
Date Published01/2005
ISSN1097-4636
Keywordsendothelialization; PEG; polyurethaneurea; vascular grafts; YIGSR
Abstract

Improved endothelialization without platelet adhesion is essential to enhance the long-term patency of synthetic vascular grafts and other blood-contacting devices. We have developed a dually modified polyurethaneurea by incorporating endothelial cell adhesive YIGSR peptide sequences as chain extenders and nonthrombogenic PEG as a soft segment (PUUYIGSR-PEG) in the polymer backbone. PUUYIGSR-PEG was successfully synthesized and characterized by proton NMR, FTIR, GPC, DSC, ESCA, and contact angle measurement. Despite having similar molecular weight, the peptide/PEG-modified polyurethaneurea (PUUYIGSR-PEG) showed superior mechanical properties compared to the control PEG-modified polyurethaneurea (PUUPPD-PEG). Virtually no platelet adhesion was observed on PUUYIGSR-PEG, while endothelial cell adhesion, spreading, and migration were significantly greater on PUUYIGSR-PEG compared to PUUPPD-PEG. Thus, this bioactive polymer may be an appropriate biomaterial for small diameter vascular grafts.

DOI10.1002/jbm.b.30135
Short TitleJ Biomed Mater Res B Appl Biomater
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