Whole-Blood Immunoassay Facilitated by Gold Nanoshell–Conjugate Antibodies

TitleWhole-Blood Immunoassay Facilitated by Gold Nanoshell–Conjugate Antibodies
Publication TypeBook Chapter
Year of Publication2005
AuthorsRosenthal, SJ, Wright, DW, Hirsch, LR, Halas, N, West, JL
Book TitleNanoBiotechnology Protocols
Series TitleMethods in Molecular Biology
Volume303
Pagination101 - 112
PublisherHumana Press
CityNew Jersey
KeywordsGold colloid; immunoassay; nanoshells; silica cores; whole blood
Abstract

In this chapter, we outline a simple procedure using gold nanoshells as a substrate for an immunoassay that is capable of detecting subnanogram levels of analyte within whole blood on the order of minutes. Unique to metallic nanoshells is their optical tunability over a large range of wavelengths. We describe the design of nanoshells that attenuate light strongly in a region of light where blood does not (i.e., the near infrared [IR]), permitting optical detection of nanoshells in whole blood. We also describe a procedure to monitor the analyte-induced aggregation of antibody-conjugated nanoshells in whole blood using near-IR light. The immunoassay is fast and specific, requires no separation/purification steps, is simple to perform (mix and sit), and uses common laboratory equipment for detection (spectrophotometer). Preparation of the antibody-nanoshell conjugates is described, along with the design and optimization of the whole-blood nanoshell-based immunoassay system.

DOI10.1385/1-59259-901-X:101
Short TitleMethods Mol Biol
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