Antibody-conjugated gold-gold sulfide nanoparticles as multifunctional agents for imaging and therapy of breast cancer

TitleAntibody-conjugated gold-gold sulfide nanoparticles as multifunctional agents for imaging and therapy of breast cancer
Publication TypeJournal Article
Year of Publication2010
AuthorsDay, ES, Bickford, LR, Slater, JH, Riggall, NS, Drezek, RA, West, JL
JournalInternational Journal of Nanomedicine
Volume5
Pagination445-454
Date Published06/2010
Keywordscancer; multiphoton microscopy; nanomedicine; photoluminescence; photothermal therapy; theranostics
Abstract

The goal of this study was to develop near-infrared (NIR) resonant gold-gold sulfide nanoparticles (GGS-NPs) as dual contrast and therapeutic agents for cancer management via multiphoton microscopy followed by higher intensity photoablation. We demonstrate that GGS-NPs exposed to a pulsed, NIR laser exhibit two-photon induced photoluminescence that can be utilized to visualize cancerous cells in vitro. When conjugated with anti-HER2 antibodies, these nanoparticles specifically bind SK-BR-3 breast carcinoma cells that over-express the HER2 receptor, enabling the cells to be imaged via multiphoton microscopy with an incident laser power of 1 mW. Higher excitation power (50 mW) could be employed to induce thermal damage to the cancerous cells, producing extensive membrane blebbing within seconds leading to cell death. GGS-NPs are ideal multifunctional agents for cancer management because they offer the ability to pinpoint precise treatment sites and perform subsequent thermal ablation in a single setting.

DOI10.2147/IJN.S10881
Short TitleInt J Nanomedicine
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